Akvarij NET - FORUM
Slatkovodna Akvaristika => Ostale slatkovodne teme => Autor teme: khodalic - Rujan 07, 2016, 19:20:39 poslijepodne
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Mikropropagacija, kao oblik vegetativnog razmnožavanja in vitro, predstavlja adekvatnu zamjenu za tradicionalne metode vegetativnog razmnožavanja
Eliminacija patogena, mogućnost vegetativnog razmnožavanja biljnih vrsta koje je inače teško razmnožavati tradicionalnim metodama i kratko vrijeme u kojem je moguće razmnožiti velik broj biljaka, glavne su prednosti i razlozi zbog kojih je mikropropagacija tako zanimljiva.
Jedne zime sam se i sam uputio u mali "Home tissue culture experiment"
Par slika
Priprema medija.
Postoje specijalizirane firme iz EU koje proizvode gotove sastojke za pripremu medija.
Na vama je samo da dodate secer u vodu te jedan od zgusnjivaca (Agar, Gellan).
Treba jos podesti pH otopine te sve sterilizirati kuhanjem u ekspres loncu.
(https://www.akvarij.net/forum/proxy.php?request=http%3A%2F%2Fi583.photobucket.com%2Falbums%2Fss279%2Fkreos123%2FHome%2520tissue%2520culture%2F20151209_155314_zpsoow16wed.jpg&hash=47f7cad80bd6891868c46843a7d3d52ea45d323d) (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20151209_155314_zpsoow16wed.jpg.html)
Dio biljke se povrsinski treba sterilizirati, u 10% otopini varikine (domestosa), kratka sterilizacija u 70% alkoholu, te ispiranje aterilnom vodom.
(https://www.akvarij.net/forum/proxy.php?request=http%3A%2F%2Fi583.photobucket.com%2Falbums%2Fss279%2Fkreos123%2FHome%2520tissue%2520culture%2FDSC007831_zpsm0dfkd0x.jpg&hash=0645f38eac68f7943b4c8fa437f23b89981be627) (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/DSC007831_zpsm0dfkd0x.jpg.html)
(https://www.akvarij.net/forum/proxy.php?request=http%3A%2F%2Fi583.photobucket.com%2Falbums%2Fss279%2Fkreos123%2FHome%2520tissue%2520culture%2F20151214_180902_zpsfn1lyix6.jpg&hash=a0c0e9eafdf686bb81c63dbb8654961bd283d6ca) (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20151214_180902_zpsfn1lyix6.jpg.html)
Prostor za "stavljanje" biljnih dijelova na hranjivu podlogu
(https://www.akvarij.net/forum/proxy.php?request=http%3A%2F%2Fi583.photobucket.com%2Falbums%2Fss279%2Fkreos123%2FHome%2520tissue%2520culture%2FDSC00836_zpsrd0itij8.jpg&hash=d41513253c868dcd2bcde1b863c9a03f168e9a64) (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/DSC00836_zpsrd0itij8.jpg.html)
Nemam laminar, uredjaj koji se puhanjem profiltriranog zraka u kontroliranim uvijetima, koristi kao prostor bez bakterija i virusa, koje znaju biti problem.
Ako i jedno zrnce prasine dospije na hranjivu podlogu prilikom stavljanja biljnog materijala na istu, moze doci do kontaminacije samog medija pa umijesto rasta i razvoja novih biljcica imate raznonrazne gljivice bakterije itd.
Moja kontaminacija je bila 50%
Musa basjoo
(https://www.akvarij.net/forum/proxy.php?request=http%3A%2F%2Fi583.photobucket.com%2Falbums%2Fss279%2Fkreos123%2FHome%2520tissue%2520culture%2FDSC00908_zpsmoordmzk.jpg&hash=6f4132da890f9e927869d651ec303e718a866ca6) (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/DSC00908_zpsmoordmzk.jpg.html)
(https://www.akvarij.net/forum/proxy.php?request=http%3A%2F%2Fi583.photobucket.com%2Falbums%2Fss279%2Fkreos123%2FHome%2520tissue%2520culture%2F20160107_171358_zpschjhydyz.jpg&hash=b74093b7e43dee109617d9d1ad664c47d36bec07) (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160107_171358_zpschjhydyz.jpg.html)
(https://www.akvarij.net/forum/proxy.php?request=http%3A%2F%2Fi583.photobucket.com%2Falbums%2Fss279%2Fkreos123%2FHome%2520tissue%2520culture%2F20160128_142848_zpsydrxad8b.jpg&hash=4853533cdb7710725d36013058501f865be1f022) (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160128_142848_zpsydrxad8b.jpg.html)
(https://www.akvarij.net/forum/proxy.php?request=http%3A%2F%2Fi583.photobucket.com%2Falbums%2Fss279%2Fkreos123%2FHome%2520tissue%2520culture%2F20160302_140510_zpsheyn5ekf.jpg&hash=489fb6ae8986a6576d738d976b0d2cc2841ef788) (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160302_140510_zpsheyn5ekf.jpg.html)
(https://www.akvarij.net/forum/proxy.php?request=http%3A%2F%2Fi583.photobucket.com%2Falbums%2Fss279%2Fkreos123%2FHome%2520tissue%2520culture%2F20160319_160629_zpsugsmvgjz.jpg&hash=8d79e3ee8a0ede8365a5f21e1ae1718a1d06a58d) (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160319_160629_zpsugsmvgjz.jpg.html)
Zavrsna faza!
Biljke "proizvedene" invitro rasle su u uvijetima velike vlage u staklenkama, te su hranjiva primala iz hranjive podloge osmozom, nema korijenja!
Kasnije se presadjuju na druge podloge s drugom koncentracijom biljnih hormona, koji poticu novi rast korijenja.
Kasnije kad izadju iz staklenke, moraju i dalje biti u uvijetima velike vlaznosti da se aklimatiziraju te da profunkcijonira korijenje.
(https://www.akvarij.net/forum/proxy.php?request=http%3A%2F%2Fi583.photobucket.com%2Falbums%2Fss279%2Fkreos123%2FHome%2520tissue%2520culture%2F20160319_175513_zpsqfnodurw.jpg&hash=5d09094ab579b4b3f5a115cf2addf3b2afb93e33) (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160319_175513_zpsqfnodurw.jpg.html)
Mislio sam nakon nekoliko dana, da su mi sve biljke propale.
Nakon 2 tjedna pocele su tjerati nove izboje
(https://www.akvarij.net/forum/proxy.php?request=http%3A%2F%2Fi583.photobucket.com%2Falbums%2Fss279%2Fkreos123%2FHome%2520tissue%2520culture%2F20160403_104023_zpsnpzoonbu.jpg&hash=8a1ca6b1c60b258857d93a3c6ead770e66deab8a) (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160403_104023_zpsnpzoonbu.jpg.html)
(https://www.akvarij.net/forum/proxy.php?request=http%3A%2F%2Fi583.photobucket.com%2Falbums%2Fss279%2Fkreos123%2FHome%2520tissue%2520culture%2F20160403_104012_zpsrifhstki.jpg&hash=8f2f6be31840f98a6b4b2efc2246dc9397422c00) (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160403_104012_zpsrifhstki.jpg.html)
(https://www.akvarij.net/forum/proxy.php?request=http%3A%2F%2Fi583.photobucket.com%2Falbums%2Fss279%2Fkreos123%2FHome%2520tissue%2520culture%2F20160508_104633_zpstzvheciv.jpg&hash=0a4bf1ae992738c4a273e3170ab77bb2fa65a19c) (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160508_104633_zpstzvheciv.jpg.html)
Uspio sam mikropropagacijom umnoziti nekoliko vrsta biljaka, vecinom Musa basjo, te Alocasia sp. te Colocasia sp.
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vrlo lijepo
jesu li troškovi bili visoki?
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vrlo lijepo
jesu li troškovi bili visoki?
Cca 100 €, ph metar, precizna vaga, fitohormoni, agar, kemikalije itd.